the one that comes up behind the train? with the barrels on the back of the train you have to unload them buy shooting the red tops of the holders on whatever relavent side that the akrid is coming up from. pretty easy when you know how just keep on going up the train after unloading the barrels.
If you're talking about the part where you have to use the railgun to kill the cat G....then just destroy its pockets of T ENG on its side and then when it comes to finish off the train in the end the game will say something like "land a killing blow" or something like that. You need to have the gun facing behind the train to kill it and make sure something is already loaded into the gun. It should be an easy single hit.
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