Orochimaru accuses Tsunade of betraying him. She responds that she wants to see both one last time, but she can't bring them back. Their dreams of protecting Konoha live onward in Naruto.
Orochimaru switches to Plan B and the great Sannin battle begins. Tsunade attacks with an ax kick and then destroys several walls with her powerful punches while Orochimaru and Kabuto strategize that relocating would be the best option, particularly since Jiraiya is here.
Shi*une, Jiraiya and Naruto arrive to find the place in ruins. Naruto sees Tsunade's coat while Shi*une's pig Ton-ton picks up her scent and points them in the right direction. Meanwhile, Kabuto pops a soldier pill and forms a seal. He then tunnels thought ground and jumps up near her, hitting her arm and abdomen so she can't use her strength. Curious, she wonders why he didn't go after her blood vessels. But Kabuto can't make his chakra reach that far into Tsunade's body during battle, so if he can take out her limbs, he can go for her throat. He is able to strike her lungs and she drops, unable to breath.
Tsunade isn't down for long before she begins her counter attack. Soon, Kabuto realizes he can't move his arm. Or more precisely, when he moves his arm, his leg moves. Taking advantage of Kabuto's disoriented state, Tsunade pounds him. She was able to slow him down with a nerve attack. By converting her chakra to an electric attack, she was able to disrupt the electrical signals the brain sends to the body by delivering electricity to 360 points throughout his nervous system. She then focuses on healing herself while Kabuto reorients himself. He pulls a kunai and is about to stab her when Jiraiya appears.
Naruto doesn't understand why Kabuto would be there, or why Tsunade would be attacking his would-be companion. He watches in horror as Kabuto stabs himself to spray blood all over Tsunade. She goes catatonic at the sight. Jiraiya explains that Kabuto was a spy for the Sound village. Kabuto chimes in that he wanted to collect data but knows that Naruto isn't as talented as Sasuke.
Kabuto continues to taunt Naruto while Jiraiya struggles to move. He Kage Bunshins but can't find an opening. Kabuto knocks Naruto back into Shi*une who shoots one of her mouth needles, but they hit his forehead protector.
Kabuto then pops a blood clotting pill and asks Orochimaru to remove the bandage from his right hand. Jiraiya commands that he will take Orochimaru while Shi*une can deal with Kabuto.