Vista shouldn't cut down GPU memory but you GPU will undoubtedly performe worse than it would using Windows XP.
If your buying a 320mb I would say definatly not but an upgrade to 500w isnt bad get a roswell brand PSU off of newegg.com they are reliable.
you should get 1 single card thats good because sli just combines processing power not memory which is much wasted in a dual setup
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