This can depend on a few things really.
Did you build the computer yourself?If so, double check that everything is seated correctly, main focus would be the extra power connectors on the graphics cards?I believe that you get a warning that they are running in low power mode if this is the case though.
What graphics drivers are you running? Have you updated your motherboard and sound card drivers as well? What size power supply do you have?
Is SLI enabled? When you update your graphics drivers it defaults to off. Right click on the desktop and select Nvidia Control Panel, if prompted, select advanced mode. On the left of this window, select "Set SLI configuration" and then press "Enable SLI technology". This might require a reboot.
I usually get my drivers from www.guru3d.com as they have everything you'll ever need, including beta drivers if you wish to try out untested drivers. It's generally better to only go with WHQL drivers though.
Also when updating graphics drivers, it's generally better to uninstall the last drivers first. You can actually do this in Control Panel -> Programs and Features. Look for Nvidia Drivers and select uninstall, this will prompt for a reboot. When Vista comes back on, decline Vista trying to install the new hardware and run the Nvidia driver install.
Have fun
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